Department of Biosciences at Novum.
Södertörns Högskola (
July, 2002-till date: Post
doctoral fellow, Karolinska Institute,
1) Studying the expression pattern of novel genes by tagging with green fluorescent protein. Making knock out mutants by a double stranded RNAi approach and study of deletion mutant by outcrossing and PCR. Co-author of manuscript entitled: The nematode unique gene, qua-1, is responsible for controlling molt in C. elegans (submitted)
2) Database mining studies to determine evolutionary profiles of Homeobox genes and to identify the entire complemet of Homeobox genes in completed genome projects. Using techniques like multiple sequence alignment, manual exon and intron boundary prediction, maximum likelihood approach for tree building, detection of conserved motifs, evolution of protein coding sequences by Positive Darwinian Evolution, I have analysed the entire homeobox gene complements of several organisms, identifying many previously unidentified genes and unrecognized motifs in the process. Co-author of manuscripts: i) Comprehensive analysis of plant homeobox genes reveals new classes, new domains and dynamic adaptations in monocots and dicots by Krishanu Mukherjee et al. (manuscript under preparation). ii) Krishanu Mukherjee and Thomas Burglin, Plant homeodomain proteins contain a sensory domain (submitted).
(Assistant Professor) in the Division of Molecular Virology, Central Potato
Project summary: Detection and strainal identification of various potato viruses and viroids through RT-PCR, nucleic acid hybridization (NASH), Western blotting, Cloning and sequencing, multiple sequence alignment, phylogenetic studies, secondary structure prediction and determination of conserved motifs within protein sequences. Plant virus purification in bulk and production of polyclonal antisera against them, purification of Immunoglobulins and preparation of ELISA kits.
1997-1998: Post Doctoral
Fellowship awarded by Indian Government Department of Biotechnology, to
work on Genetic Transformation of potato at
Project summary: Osmotin gene conferring resistance to Phytophthora infestans was initially subcloned and then finally cloned into Agrobacterium Plant Expression vector (PGA643). Late blight susceptible potato variety (Darjeeling Red Round ) was transformed and stably transformed lines were found by PCR and Southern hybridization.
1991-1996: Doctoral Studies
(Ph.D.) in Plant Pathology, Indian Agricultural Research Institute, New
Title of Ph.D.thesis: Technologies for field application of an
effective biocontrol agent Aspergillus
Publication: Krishanu Mukherjee and Bineeta Sen. 1998. Biological control of Fusarium wilt of muskmelon by formulations of
Courses undertaken during Doctoral program:
Grades obtained: 3.64/4.00
1989-1990: Masters (M.Sc.
in Agriculture) from Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia,
Grades obtained: 3.72/4.00
Courses undertaken during Masters program: Virology, Bacteriology, Fungal Pathology, Entomology, Nematology, Biochemistry, Molecular Biology.
1984-1988: Bachelors (B.Sc.
in Agriculture (Hons.)) from Bidhan Chandra Krishi Viswavidyalaya, Kalyani,
Marks obtained: 78.5%
Courses in Bachelors degree included:
Statistics, Mathematics, Physics, Chemistry, Zoology, Botany, Genetics, Biochemistry, Microbiology, Entomology, Virology, Bacteriology, Fungal Pathology, Plant pathology and Agriculture.
2001 September to 2002 February: Advanced certification course in Bioinformatics (Sequence Alignment (BLAST, FASTA)
Nucleic acid / protein sequence analysis (EMBOSS, Online tools),
Multiple alignments for Structural Functional, or Phylogenetic analysis (CLUSTAL X, NJPLOT, Claris Draw, Free hand), Secondary structure prediction (Manually, J-pred, Chou-Fasman, Garnier), Homology modeling (Swiss PDB Viewer, Swiss-Model, Modeller, Procheck, Verify 3D), Threading methods for protein structure prediction etc. )
Diploma in Web and E-Commerce Technology (C,
Advance Java, HTML, Active Server Pages (ASP), Oracle 8i) from STG
1999-2000: Certificate course in C language
Proficiency in Perl language
LIST OF PUBLICATION:
Krishanu Mukherjee, Yogita
Verma, S.K.Chakrabarty, and S.M. Paul Khurana. 2004. Phylogenetic analysis of 5¢-UTR and P1 protein of common strain
of Potato Virus Y reveals its possible introduction in
Krishanu Mukherjee, Yogita Verma, S.K.Chakrabarty, M.N.Singh and S.M. Paul Khurana. 2003. Cloning and sequencing of coat protein gene of an Indian potato leaf roll virus (PLRV) isolate and its similarity with other members of Luteoviridae. Virus Genes 26 (3) 247-253.
v)Verma, Yogita, Khurana, S. M. Paul, Mohan, Jitendra, Sood, Shivali, and Mukherjee, Krishanu 2003. Development of a non- radioactive probe for detection of potato virus Y. Journal of Indian Potato Association. 30 (1-2) : 111-112
Krishanu Mukherjee and Bineeta
Sen. 1998. Biological control of Fusarium wilt of muskmelon by formulations of